RNA Structure and RNA–Protein Interactions in Purified Yeast U6 snRNPs
نویسندگان
چکیده
منابع مشابه
RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.
The U6 small nuclear RNA (snRNA) undergoes major conformational changes during the assembly of the spliceosome and catalysis of splicing. It associates with the specific protein Prp24p, and a set of seven LSm2p-8p proteins, to form the U6 small nuclear ribonucleoprotein (snRNP). These proteins have been proposed to act as RNA chaperones that stimulate pairing of U6 with U4 snRNA to form the int...
متن کاملDirect probing of RNA structure and RNA-protein interactions in purified HeLa cell's and yeast spliceosomal U4/U6.U5 tri-snRNP particles.
The U4/U6.U5 tri-snRNP is a key component of spliceosomes. By using chemical reagents and RNases, we performed the first extensive experimental analysis of the structure and accessibility of U4 and U6 snRNAs in tri-snRNPs. These were purified from HeLa cell nuclear extract and Saccharomyces cerevisiae cellular extract. U5 accessibility was also investigated. For both species, data demonstrate t...
متن کاملRNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
BACKGROUND The dynamic rearrangements of RNA structure which occur during pre-mRNA splicing are thought to be mediated by members of the DExD/H-box family of RNA-dependent ATPases. Although three DExD/H-box splicing factors have recently been shown to unwind synthetic RNA duplexes in purified systems, in no case has the natural biological substrate been identified. A duplex RNA target of partic...
متن کاملStructure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy.
Protein components of the U6 snRNP (Prp24p and LSm2-8) are thought to act cooperatively in facilitating the annealing of U6 and U4 snRNAs during U4/U6 di-snRNP formation. To learn more about the spatial arrangement of these proteins in S. cerevisiae U6 snRNPs, we investigated the structure of this particle by electron microscopy. U6 snRNPs, purified by affinity chromatography and gradient centr...
متن کاملPrimary and hypothetical secondary structure of Drosophila melanogaster U6 RNA.
A comparison of the nucleotide sequence of Drosophila U6 RNA with that of rat (6) reveals a homology of 90.7%. Of the 10 differences, 4 represent sugar and base modifications (positions 64, 69, 71, 73) while 6 are nucleotide replacements (positions 4, 6,18,65,86,102). Multiple alignements show that the nucleotide sequences of broad bean (7) and Physarum polycephalum U6 snRNA (8) have much weake...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2006
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2005.12.013